The application note details a method for quantifying proteins using the FL 6500 Fluorescence Spectrometer and NanoOrange dye. This approach is highlighted for its sensitivity and specificity, especially suitable for detecting low-abundance proteins in complex biological samples.
The document outlines the experimental setup, including reagent preparation and fluorescence measurement parameters. Results demonstrate the method's reliability and accuracy through the creation of standard curves and validation across multiple tests. It concludes that the NanoOrange fluorescent-based method, coupled with the FL 6500's advanced optics, offers a simple, sensitive, and efficient solution for protein quantification, beneficial for laboratories operating in regulated environments.