Sugars are the most important component of soft drinks, candies, and sweets. Therefore, sugars quantitation in syrups used for manufacturing or ready products is of extreme importance. In the USA, not only traditional saccharose is in use, but high fructose corn syrup, the mix of fructose and glucose has the largest share of the market. Maltose is also in use in some food products. The PerkinElmer introduced Epic Amino HD column and LC300 UHPLC System which could be equipped with a Refractive Index detector. We are evaluating the suitability of this column and instrument for the determination of sugar content in soft drinks.