LC 300 HPLC & UHPLC System | PerkinElmer
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LC 300 HPLC and UHPLC Systems

For laboratories looking to achieve the highest levels of versatility without compromising on productivity, PerkinElmer LC 300 HPLC and UHPLC Systems deliver the flexibility, performance, and efficiency you need for even the most challenging analytical demands. With fully customizable configurations and accessories, each LC 300 HPLC and UHPLC Systems is intuitive and designed to meet high-throughput lab requirements, with no additional burden on lab staff. PerkinElmer SimplicityChrom CDS Software manages the complete workflow and with the LC 300 HPLC and UHPLC Systems delivers an innovative user experience with intuitive functions and automated processes.

Part Number
Model Name
N2550110
LC 300 HPLC System
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N2550150
LC 300 Analytical HPLC System
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N2550120
LC 300 UHPLC System
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Overview

The PerkinElmer LC 300 HPLC and UHPLC System has been designed to increase laboratory productivity and throughput, even in the face of challenging budget and staffing situations. Paired with the intuitive SimplicityChrom Chromatography Data System (CDS) Software, our LC 300 HPLC and UHPLC System are the ideal solution for labs seeking to maximize their liquid chromatography experience. The LC 300 HPLC and UHPLC System offers superior performance and flexibility throughout its intelligent design:

  • Robust and precise autosampler with integrated column oven and preheater, as well as a high visibility color LCD display showing full instrument system status, current queue and sample progress.
  • Optional external Peltier column oven for ultra-precise heating and cooling, for sample integrity and exceptionally accurate results.
  • Patented Intermediate Loop Decompression (ILD) valve ensures the injection of an accurate and reproducible sample volume, while also increasing the lifetime of columns used in analysis.
  • Automated, onboard sample dilution and derivatization capabilities simplify the front-end analytical processes, while also ensuring reproducibility and precision.
  • 6k psi analytical pump for reliable and robust performance day after day. 10k psi solvent delivery pump for high-end HPLC functionality, delivering repeatable solvent delivery at a wide range of flow rates and pressures. Powerful 18k psi pump for ultra-precise UHPLC analyses, delivering repeatable solvent delivery at a wide range of flow rates and pressures.
  • Automatic priming design allows the system to achieve full self-priming capabilities, automating the otherwise cumbersome priming process utilized in many other systems.
  • Built-in solvent degassing technology simplifies the analytical process, and obviates the need for a separate, external degassing module.
  • Portfolio of five available detectors, which can be used individually or in sequence.
    • Photodiode array (PDA)
    • Multiwavelength UV/Vis (MWD)
    • Single wavelength UV/Vis
    • Refractive index (RI)
    • Fluorescence (FL)
  • Efficient and powerful instrument control with SimplicityChrom CDS Software, allowing customizable workflows and experiences and supporting compliance with 21 CFR Part 11 requirements.

Specifications

Model Name LC 300 UHPLC System

Configurations

Depth

22 inches

Weight 183 pounds (single stack with one detector and standalone solvent organizer) or 260 pounds maximum (dual stack with external oven)
Width 13.5 inches maximum per component
Resources, Events & More
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Application Brief

HPLC Analysis of Lamotrigine Using LC 300 in Accordance With USP

Lamotrigine is an antiepileptic drug in the phenyl triazine class used in the treatment of both epilepsy and as a mood stabilizer for bipolar disorder. It is the first medication since lithium to be granted US FDA approval for maintenance treatment of bipolar type 1, and is approved for use in more ...

PDF 370 KB
HPLC Analysis of Omeprazole Using LC 300 in Accordance with USP

The World Health Organization regards omeprazole among its ‘List of Essential Medicines’ which outlines the most effective and safest medicines required by a country's healthcare system to meet its needs. The United States Pharmacopeia (USP) closely monitors the production of these drugs and provide ...

PDF 274 KB
HPLC Analysis of Pregabalin Using LC 300 in Accordance With USP

Pregabalin is an anti-epileptic drug that works by slowing down impulses in the brain that cause seizures. It can also be utilized to treat fibromyalgia, or nerve pain in people with diabetes, herpes zoster, or spinal cord injury owing to its ability to affect chemicals in the brain that send pain s ...

PDF 292 KB
HPLC Analysis of Triamcinolone Acetonide Purity Using LC 300 in Accordance With USP

This Application Brief demonstrates the ability of PerkinElmer's LC 300 instrument platform, and SimplicityChrom™ chromatographic data system (CDS) software to successfully meet the United States Pharmacopeia (USP) performance requirements for the HPLC analysis of Triamcinolone Acetonide purity. ...

PDF 322 KB
HPLC Assay of Risperidone Impurities Using LC 300 in Accordance With USP

This Application Brief demonstrates the ability of PerkinElmer's LC 300 instrument platform, and SimplicityChrom™ chromatographic data system (CDS) software to successfully meet the United States Pharmacopeia (USP) performance requirements for the HPLC analysis of Risperidone impurities. ...

PDF 316 KB
HPLC Assay of Risperidone Using LC 300 in Accordance With USP

Risperidone is an antipsychotic medicine that works by changing the effects of chemicals in the brain. The methods for assessing Risperidone characteristics are described in the United States Pharmacopeia and rely on chromatographic techniques. In this application brief, we demonstrate the suitabili ...

PDF 285 KB
HPLC Assay of Triamcinolone Acetonide Using LC 300 in Accordance With USP

This Application Brief demonstrates the ability of PerkinElmer's LC 300 instrument platform, and SimplicityChrom™ chromatographic data system (CDS) software to successfully meet the United States Pharmacopeia (USP) performance requirements for the HPLC analysis of Triamcinolone Acetonide. ...

PDF 353 KB
Qualitative Identification of Tortoise-Shell Glue Using LC/MS/MS

This application note describes a regulatory LC/MS/MS method for the qualitative identification of tortoise-shell glue, an important Traditional Chinese Medicine (TCM) known for its hemopoietic and hemostatic properties.

Application Brief
Oleanlic Acid and Ursonic Acid Analysis of Chaenomelis Fructus by LC-UV

This application note outlines an LC-UV method for analyzing oleanolic acid and ursonic acid in Chaenomelis fructus, known for its medicinal properties.

Application Brief
Workflow for Qualitative Identification of Donkey-Hide Gelatin

This application note discusses a qualitative LC/MS/MS workflow for identifying donkey-hide gelatin, a valuable traditional Chinese medicinal product.

Application Brief

Application Note

Analysis of 16 Cannabinoids Using the PerkinElmer LC 300 HPLC System with PDA Detection

Accurate identification and quantification of cannabinoid content is a critical step in the process to bringing safe and high quality cannabis flower and fortified products to market. As the legalization of recreational cannabis use continues throughout the world, fast and efficient liquid chromatog ...

PDF 1 MB
Analysis of Common Preservatives in Personal Care Products by HPLC with UV Detection

Parabens are utilized as an antimicrobial preservative in many commercial offerings including cosmetics, personal care products and foods, and offer both chemical and thermal stability to products. Although parabens increase the safety of products, recent studies have suggested that parabens may exh ...

PDF 1 MB
Analysis of Phytoestrogen Isoflavones in Dietary Supplements by HPLC/UV

Isoflavones are water-soluble compounds found in many plant and food sources. Designated as phytoestrogens, isoflavones are structurally similar to to the female hormone estrogen, and have been shown to exhibit a number of beneficial impacts to human health, such as a reduction in the development of ...

PDF 1 MB
Analysis of Saccharides in High Fructose Corn Syrup

High fructose corn syrup (HFCS) as a substitute for sucrose in a wide variety of products, including baked goods, sauces, beverages, canned fruits and dairy products. HFCS is produced from regular corn syrup, with an enzymatic process that isomerizes some of the corn syrup's glucose into fructose. T ...

PDF 1 MB
Analysis of Sugars in Maple Syrup by HILIC with Refractive Index Detection

Maple syrup, a common natural sweetener, is consumed regularly by households around the world. Quantification of sugar content in maple syrup is paramount to satisfy product labeling requirements and ensure label-claim accuracy. Although sucrose is the primary sugar component in maple syrup, a compl ...

PDF 1 MB
HPLC: Analysis of Acrylate Monomers

Acrylate polymers are utilized for a host of applications due to their varied physical properties and ease of synthesis. These acrylates are sought after specifically for coatings, adhesives, sealants and elastomers (CASE) applications because of their stability, high heat resistance, excellent weat ...

PDF 1 MB
HPLC: Analysis of mRNA with PDA Detection

The recent emergence of mRNA-based vaccines and therapies has made clear the need for reliable analytical methods that can successfully identify, characterize, and quantify mRNA. This chemical instability demands a deeper understanding of the mechanisms by which mRNA degrades, which can inform the d ...

PDF 816 KB
HPLC: Rapid Quantitation of Vitamins in Biological Fluids using HPLC and IVD-CE Certified Kits

Vitamins are involved in a variety of different physiological processes and must be assimilated with the daily nutrition as they cannot be synthesized within the human body. The biochemical functions of vitamin C (ascorbic acid) are mainly linked to its antioxidant and radical scavenger properties. ...

PDF 597 KB
The Analysis of Lactose in Milk and Cheese Products by HPLC with RI Detection

This application note demonstrates a method for the routine sample preparation and quantitative determination of lactose in mozzarella and ricotta cheeses and milk. As the monosaccharaides glucose and galactose can be hydrolytically or enzymatically produced from lactose, these two sugars were also ...

PDF 1 MB
Glutaraldehyde Analysis in Bioprosthetic Heart Valve by LC-UV

This application note presents a detailed LC-UV workflow for analyzing glutaraldehyde in bioprosthetic heart valves using the PerkinElmer LC 300 system.

Application Note
Paeoniflorin Analysis in Paeoniae Radix Alba by LC-UV

This application note describes the quantification of paeoniflorin in Paeoniae radix alba using a PerkinElmer LC 300 UHPLC system. By employing a polar column, the method enhances separation performance and meets the Chinese Pharmacopoeia specifications.

Application Note
Rapid Analysis of Gelatinous Traditional Chinese Medicine

This document details a rapid LC/MS/MS workflow for analyzing gelatinous traditional Chinese medicines using the PerkinElmer QSight LX50 UHPLC system.

Application Note
Analysis of Aflatoxins in Traditional Chinese Medicine Coix Seed Using LC/MS/MS

This application note presents a sensitive LC/MS/MS method for detecting aflatoxins in Coix seed, a traditional Chinese medicine. The analysis, conducted on a PerkinElmer LC 300 system, meets the stringent sensitivity and accuracy requirements of the Chi

Application Note
Characteristic Components Analysis in Lonicerae Japonica Flos by LC-UV

This application brief details a comprehensive LC-UV method for analyzing multiple characteristic components in Lonicerae japonica flos, used for treating inflammation and infectious diseases.

Application Note
Chlorogenic Acid Analysis in Children Throat-Reliever Granule by LC-UV

This application note presents an LC-UV workflow for the quantification of chlorogenic acid in children's throat-reliever granules, a traditional Chinese medicine. The analysis was conducted using a PerkinElmer LC 300 UHPLC system with a C18 column.

Application Note
HPLC Analysis of Oleanolic Acid and Ursonic Acid in Eriobotrya Japonica Leaf

This document outlines a robust LC-UV method for quantifying the triterpene acids oleanolic acid and ursonic acid in Eriobotrya japonica leaf, a traditional Chinese medicinal material. Utilizing a PerkinElmer LC 300 system with a C18 column, the method a

Application Note
Ferulic Acid Analysis in Angelica Sinensis Radix by HPLC

This application note describes a detailed LC-UV workflow for analyzing ferulic acid, an important phenolic acid used for quality assessment, in Angelica sinensis (A. sinensis). The analysis was performed using a PerkinElmer LC 300 system equipped with a

Application Note

Article

Best Practices for HPLC to UHPLC Method Transfer

Best Practices for HPLC to UHPLC Method Transfer Converting a method from HPLC to UHPLC has a number of benefits, as outlined in the first 2 articles in our series, including faster analytical separations, better peak resolution and lower long-term costs

Article

Brochure

Chromatography Solutions for Soft Drink Analysis

Monitoring the consistency and quality in soft drinks is essential to ensure a positive brand image and a loyal customer base. Our full portfolio of chromatography solutions, including LC, GC and GC/MS, enables you to meet – and even exceed – your quality control goals with rapid, intuitive, and acc ...

PDF 1 MB
LC 300 HPLC and UHPLC Systems Interactive Brochure

For laboratories looking to achieve the highest levels of versatility without compromising on productivity, the LC 300 HPLC and UHPLC Systems deliver the flexibility, performance, and efficiency you need for even the most challenging analytical demands. With fully customizable configurations and acc ...

PDF 2 MB
Advanced Solutions for Biofuels Development and Testing

Download this interactive brochure to see all our biofuel lab solutions and supporting services. Maximize your laboratory's prospects in biofuels production and testing with PerkinElmer’s analytical solutions that can help you achieve high product qualit

Brochure

Flyer

Semiconductor & Electronics Analysis Overview Flyer

Download this Flyer for an overview of PerkinElmer's analytical solutions for the Semiconductor industry. This 2-page flyer outlines semiconductor and microelectronic workflows and applications, and the precise and reliable PerkinElmer solutions to address them - including instrumentation, consumabl ...

PDF 1 MB
SimplicityChrom CDS

SimplicityChrom™ is a modern, intuitive and flexible CDS software platform that enables users to efficiently control their instrument, process data, and generate results. Pair this functionality with the peace of mind of 21 CFR Part 11 compliance and industry-leading service and support, and you’re ...

PDF 1 MB

Guide

How to Guide for HPLC: Choosing the Right Detector

There are many characteristics to consider when choosing a detector, therefore several detectors have been developed over time to address particular challenges. It is important to consider the chemical nature of the analytes of interest and any potential interferences, when choosing a detector. Addi ...

PDF 766 KB

Product Note

LC 300 HPLC and UHPLC Systems - Product Note - Redefining LC Versatility, Maximizing Lab Productivity

For laboratories looking to achieve the highest levels of versatility without compromising on productivity, the LC 300 HPLC and UHPLC Systems deliver the flexibility, performance, and efficiency you need for even the most challenging analytical demands. With fully customizable configurations and acc ...

PDF 785 KB

Technical Note

PerkinElmer LC 300 Autosampler Intermediate Loop Decompression (ILD) Injection Valve

The injection of an accurate and reproducible sample volume into a UHPLC system is challenging, but essential to ensuring a high quality analysis. The patented PerkinElmer Intermediate Loop Decompression (ILD) valve, featured in the LC 300 Autosampler, is a 7-port valve with a strategically placed r ...

PDF 849 KB

Video

Performance and Productivity with the LC 300 HPLC & UHPLC Systems

For laboratories focused on achieving high levels of efficiency, without compromise on productivity, the PerkinElmer LC 300 HPLC and UHPLC System offers performance and productivity even for the most challenging analytical demands. Discover more.

Video
HPLC Columns: The Foundation of Great Chromatography

The foundation of great Chromatography starts with selecting the right column. Our LC column portfolio brings you new opportunities for full performance-boosting LC workflow solutions, ensuring that you have the optimum column for your application.

Video

Webinars

An Introduction to LC-MS/MS

This presentation is the first in a series that will introduce you to the highly useful analytical technique - High Performance Liquid Chromatography - Mass Spectrometry (LC-MS).

Webinars
Chromatography Simplified – Improving LC Workflow Efficiency and Productivity

Watch our on-demand webinar to discover the difference that a focus on improving the user experience will have on your workflows and productivity.

Webinars