Introduction
This application note demonstrates a fast, sensitive, and highly robust LC‑MS/MS solution for honey authenticity testing through targeted quantification of 3‑methoxytyramine (3‑MT), a specific marker of beet‑sugar feeding. Using the QSight® 500 LC‑MS/MS system with EDIT‑ESI™ and HSID™ StayClean™ technologies, the method overcomes the analytical challenges of sugar‑rich honey matrices without relying on solid‑phase extraction (SPE) or isotopically labeled internal standards.
A simplified Blend‑and‑Inject workflow enables reliable sub‑ppb detection, excellent precision, and long‑term system stability, making the approach well suited for routine, high‑throughput commercial and regulatory honey testing laboratories.
Key Highlights
- Fast, simple prep – Blend‑and‑Inject workflow cuts sample prep to <10 minutes with no SPE or labeled standards.
- High matrix robustness – EDIT‑ESI™ and HSID™ technologies ensure stable performance in sugar‑rich honey matrices.
- Targeted authenticity testing – Sensitive LC‑MS/MS detection of 3‑methoxytyramine enables confident beet‑sugar feeding identification.
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